bd40bc7c7a Data is presented as mean SD. Diabetes 62: 98792. siRNA transfection of 3T3-L1 adipocytes and lipogenesis assay For detailed description of siRNA and lipogenesis experiments see Methods S1. Clinic (Spay/Neuter Incentive Program), more than 10,000 dogs and cats are spayed or neutered each year helping reduce pet overpopulation and eliminate future animal suffering. Dev Cell 13: 391404. (2005) Epigenetic differences arise during the lifetime of monozygotic twins.
doi: 10.2337/db11-0737 View Article PubMed/NCBI Google Scholar 36. Watson PJ, Fairall L, Schwabe JW (2012) Nuclear hormone receptor co-repressors: structure and function. Slentz CA, Houmard JA, Kraus WE (2009) Exercise, abdominal obesity, skeletal muscle, and metabolic risk: evidence for a dose response. Among all 476,753 CpG sites analyzed on the Infinium HumanMethylation450 BeadChip and passing QC, 1,351 sites mapped to 53 genes suggested to contribute to obesity in the review by McCarthy, and 1,315 sites mapped to 39 genes suggested to contribute to T2D . TSS, proximal promoter, defined as 200 bp (basepairs) or 1500 bp upstream of the transcription start site; UTR, untranslated region; CpG island, 200 bp (or more) stretch of DNA with a C+G content of 50% and an observed CpG/expected CpG in excess of 0.6; Shelf, regions flanking island shores, i.e., covering 20004000 bp distant from the CpG island; Shore: the flanking region of CpG islands, 02000 bp. doi: 10.2337/db12-0548 View Article PubMed/NCBI Google Scholar 53. Irizarry RA, Ladd-Acosta C, Wen B, Wu Z, Montano C, et al. (2010) Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distribution. Genome Res 20: 434439. Secondly we tested for association between age and the level of DNA methylation in the 31 individuals included at baseline in this study, representing a more valid age range (3045 years) and tissue for the current hypothesis.